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How to make siRNA

The simplest and easist way to make siRNA is to buy ready-made products. Before you are taking action you need some knowlege about RNAi to decide which one to have. Here are some tips for making your decision.

Make siRNA yourself, in fact is straightforward process. There are 5 ways to make your own siRNA:

• Chemical synthesis
• In vitro transcription
• RNase III Digestion of dsRNA
• shRNA expression plasmid or viral vector
• PCR-derived siRNA expression cassette

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